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Issue 54, 2019
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Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

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Abstract

The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.

Graphical abstract: Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

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Supplementary files

Article information


Submitted
06 Aug 2019
Accepted
20 Sep 2019
First published
03 Oct 2019

This article is Open Access

RSC Adv., 2019,9, 31511-31516
Article type
Paper

Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

F. Allabush, P. M. Mendes and J. H. R. Tucker, RSC Adv., 2019, 9, 31511
DOI: 10.1039/C9RA07570D

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