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Issue 61, 2019
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Function of pathway specific regulators in the ACE1 and pyrichalasin H biosynthetic gene clusters

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Abstract

Ectopic expression of BC1 which encodes a putative pathway specific transcription factor from the ACE1 biosynthetic gene cluster of the rice pathogen Pyricularia oryzae Guy11 did not lead to the production of ACE1-related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of pyiR which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in Magnaporthe grisea NI980 led to the apparent up-regulation of the pyi cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.

Graphical abstract: Function of pathway specific regulators in the ACE1 and pyrichalasin H biosynthetic gene clusters

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Supplementary files

Article information


Submitted
03 Sep 2019
Accepted
28 Oct 2019
First published
04 Nov 2019

This article is Open Access

RSC Adv., 2019,9, 35797-35802
Article type
Paper

Function of pathway specific regulators in the ACE1 and pyrichalasin H biosynthetic gene clusters

V. Hantke, C. Wang, E. J. Skellam and R. J. Cox, RSC Adv., 2019, 9, 35797
DOI: 10.1039/C9RA07028A

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