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Issue 14, 2019, Issue in Progress
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A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

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Abstract

A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.

Graphical abstract: A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

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Publication details

The article was received on 10 Jan 2019, accepted on 25 Feb 2019 and first published on 12 Mar 2019


Article type: Paper
DOI: 10.1039/C9RA00210C
RSC Adv., 2019,9, 7955-7960
  • Open access: Creative Commons BY-NC license
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    A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

    H. Zhang, L. Xu, W. Li, W. Chen, Q. Xiao, J. Huang, C. Huang, J. Sheng and X. Song, RSC Adv., 2019, 9, 7955
    DOI: 10.1039/C9RA00210C

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