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Issue 8, 2019, Issue in Progress
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High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting

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Abstract

A high-throughput screening system based on droplet microfluidic sorting was developed and employed for screening of high lactic acid-producing Bacillus coagulans. In this system, water-in-oil-in-water (W/O/W) droplets, which were ∼12 pL in volume were used as picoliter-reactors for lactic acid fermentation. A fluorescent sensor was developed and used for monitoring pH which indicated the production of lactic acid. After fermentation, fluorescence activated cell sorting was performed with high sensitivity and speed. Using this microfluidic high-throughput screening system, we found a mutant with a yield of 76 g L−1 lactic acid which was 52% higher than its parent strain with a screening throughput exceeding 106 clones per h.

Graphical abstract: High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting

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Supplementary files

Article information


Submitted
25 Nov 2018
Accepted
04 Jan 2019
First published
05 Feb 2019

This article is Open Access

RSC Adv., 2019,9, 4507-4513
Article type
Paper

High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting

X. Zhu, X. Shi, S. Wang, J. Chu, W. Zhu, B. Ye, P. Zuo and Y. Wang, RSC Adv., 2019, 9, 4507
DOI: 10.1039/C8RA09684H

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