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pH-Responsive protein nanoparticles via conjugation of degradable PEG to the surface of cytochrome c

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Abstract

Proteins represent a versatile biopolymer material for the preparation of nanoparticles. For drug delivery applications an acid-triggered disassembly and payload release is preferred. Herein, we present a protein nanoparticle system based on cytochrome c, which is surface-modified with acid-degradable polyethylene glycol (PEGylation). pH-Sensitivity was obtained through vinyl ether moieties distributed in the polyether backbone. When PEGylated, cytochrome c shows a different solubility behaviour in organic solvents, which allows for particle preparation using an emulsion-based solvent evaporation method. The resulting particles are stable under physiological conditions but degrade at acidic pH values. Fluorescence-labelled dextran was successfully encapsulated as a hydrophilic model payload in these degradable nanoparticles and a release under acidic conditions was observed.

Graphical abstract: pH-Responsive protein nanoparticles via conjugation of degradable PEG to the surface of cytochrome c

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Publication details

The article was received on 02 Aug 2019, accepted on 16 Oct 2019 and first published on 17 Oct 2019


Article type: Paper
DOI: 10.1039/C9PY01162E
Polym. Chem., 2019, Advance Article

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    pH-Responsive protein nanoparticles via conjugation of degradable PEG to the surface of cytochrome c

    E. Steiert, J. Ewald, A. Wagner, U. A. Hellmich, H. Frey and P. R. Wich, Polym. Chem., 2019, Advance Article , DOI: 10.1039/C9PY01162E

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