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Issue 23, 2019
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Leak-free million-fold DNA amplification with locked nucleic acid and targeted hybridization in one pot

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Abstract

An isothermal cascade reaction that exponentially amplifies pre-designed, single-stranded DNA as a sensor and signal amplifier module for DNA-based computing and molecular robotics was developed. Taking advantage of the finding that locked nucleic acid can suppress problematic ab initio DNA synthesis, up to million-fold amplification rates and concurrent hybridization were achieved at a physiological temperature in a single reactor. Although the effect of locked nucleic acid introduction to the templates was complicated, undesired leak DNA amplification was generally suppressed in the amplification reaction for distinct DNA sequences. The present reaction that senses one DNA as an input and generates a large amount of another DNA as an output, exhibiting a high correlation between the molecular concentration and the amplification time, is applicable for nucleic acid quantification.

Graphical abstract: Leak-free million-fold DNA amplification with locked nucleic acid and targeted hybridization in one pot

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Supplementary files

Article information


Submitted
03 Mar 2019
Accepted
27 Mar 2019
First published
09 Apr 2019

Org. Biomol. Chem., 2019,17, 5708-5713
Article type
Paper
Author version available

Leak-free million-fold DNA amplification with locked nucleic acid and targeted hybridization in one pot

K. Komiya, M. Komori, C. Noda, S. Kobayashi, T. Yoshimura and M. Yamamura, Org. Biomol. Chem., 2019, 17, 5708
DOI: 10.1039/C9OB00521H

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