Jump to main content
Jump to site search

Issue 41, 2019

Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo

Author affiliations

Abstract

Nanosystems are able to enhance bone regeneration, a complex process requiring the mutual interplay between immune and skeletal cells. Activated monocytes can communicate pro-osteogenic signals to mesenchymal stem cells and promote osteogenesis. Thus, the activation of monocytes is a promising strategy to improve bone regeneration. Nanomaterials specifically selected to provoke immune-mediated bone formation are still missing. As a proof of concept, we apply here the intrinsic immune-characteristics of graphene oxide (GO) with the well-recognized osteoinductive capacity of calcium phosphate (CaP) in a biocompatible nanomaterial called maGO-CaP (monocytes activator GO complexed with CaP). In the presence of monocytes, the alkaline phosphatase activity and the expression of osteogenic markers increased. Studying the mechanisms of action, we detected an up-regulation of Wnt and BMP signaling, two key osteogenic pathways. The role of the immune activation was evidenced by the over-production of oncostatin M, a pro-osteogenic factor produced by monocytes. Finally, we tested the pro-osteogenic effects of maGO-CaP in vivo. maGO-CaP injected into the tibia of mice enhanced local bone mass and the bone formation rate. Our study suggests that maGO-CaP can activate monocytes to enhance osteogenesis ex vivo and in vivo.

Graphical abstract: Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo

Supplementary files

Article information


Submitted
09 May 2019
Accepted
22 Jul 2019
First published
30 Jul 2019

Nanoscale, 2019,11, 19408-19421
Article type
Paper

Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo

V. Bordoni, G. Reina, M. Orecchioni, G. Furesi, S. Thiele, C. Gardin, B. Zavan, G. Cuniberti, A. Bianco, M. Rauner and L. G. Delogu, Nanoscale, 2019, 11, 19408 DOI: 10.1039/C9NR03975A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements