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Issue 2, 2019
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Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

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Abstract

We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.

Graphical abstract: Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

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Publication details

The article was received on 08 Nov 2018, accepted on 14 Nov 2018 and first published on 21 Nov 2018


Article type: Communication
DOI: 10.1039/C8NA00331A
Nanoscale Adv., 2019,1, 532-536
  • Open access: Creative Commons BY license
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    Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

    C. Jumeaux, E. Kim, P. D. Howes, H. Kim, R. Chandrawati and M. M. Stevens, Nanoscale Adv., 2019, 1, 532
    DOI: 10.1039/C8NA00331A

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