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Issue 20, 2019
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Microfluidic quantification and separation of yeast based on surface adhesion

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Abstract

Fungal adhesion is fundamental to processes ranging from infections to food production to bioengineering. Yet, robust, population-scale quantification methods for yeast surface adhesion are lacking. We developed a microfluidic assay to discriminate and separate genetically-related yeast strains based on adhesion strength, and to quantify effects of ionic strength and substrate hydrophobicity on adhesion. This approach will enable the rapid screening and fractionation of yeast based on adhesive properties for genetic protein engineering, anti-fouling surfaces, and a host of other applications.

Graphical abstract: Microfluidic quantification and separation of yeast based on surface adhesion

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Supplementary files

Article information


Submitted
22 Mar 2019
Accepted
26 Aug 2019
First published
16 Sep 2019

This article is Open Access

Lab Chip, 2019,19, 3481-3489
Article type
Paper

Microfluidic quantification and separation of yeast based on surface adhesion

K. Reinmets, A. Dehkharghani, J. S. Guasto and S. M. Fuchs, Lab Chip, 2019, 19, 3481
DOI: 10.1039/C9LC00275H

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    [Original citation] - Published by The Royal Society of Chemistry.

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