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Issue 10, 2019
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Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

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Abstract

Isolation of circulating tumor cells (CTCs) from blood provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancer. The roles and clinical values of CTCs are under intensive investigation, yet most studies are limited by technical challenges in the comprehensive enrichment of intact and viable CTCs with minimal white blood cell (WBC) contamination. Here, we report a novel method based on contrast of cell magnetization in biocompatible ferrofluids (a colloidal magnetic nanoparticle suspension), termed as integrated ferrohydrodynamic cell separation (iFCS), that enriches CTCs in a tumor antigen-independent and cell size variation-inclusive manner, achieves a high throughput (12 mL h−1), high recovery rate (99.08% at down to ∼10 cells per mL spike ratio), and low WBC contamination (533 cells for every one milliliter blood processed) and is biocompatible. This method will enable large cohort research to define the clinical and diagnostic value of CTC subtypes.

Graphical abstract: Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

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Publication details

The article was received on 28 Feb 2019, accepted on 16 Apr 2019 and first published on 23 Apr 2019


Article type: Paper
DOI: 10.1039/C9LC00210C
Lab Chip, 2019,19, 1860-1876

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    Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

    W. Zhao, Y. Liu, B. D. Jenkins, R. Cheng, B. N. Harris, W. Zhang, J. Xie, J. R. Murrow, J. Hodgson, M. Egan, A. Bankey, P. G. Nikolinakos, H. Y. Ali, K. Meichner, L. A. Newman, M. B. Davis and L. Mao, Lab Chip, 2019, 19, 1860
    DOI: 10.1039/C9LC00210C

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