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Issue 14, 2019
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Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

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Abstract

We report a microfluidic fluorescence activated cell-sorting (μFACS) device that employs traveling surface acoustic waves (TSAW) to sort cells at rates comparable to conventional jet-in-air FACS machines, with high purity and viability. The device combines inertial flow focusing and sheath flow to align and evenly space cells, improving the sorting accuracy and screening rate. We sort with an interdigital transducer (IDT) whose tapered geometry allows precise positioning of the TSAW for optimal cell sorting. We sort three different cell lines at several kHz, at cell velocities exceeding one meter per second, while maintaining both sorting purity and cell viability at around 90% simultaneously.

Graphical abstract: Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

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Article information


Submitted
15 Feb 2019
Accepted
04 Jun 2019
First published
13 Jun 2019

Lab Chip, 2019,19, 2435-2443
Article type
Paper
Author version available

Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

K. Mutafopulos, P. Spink, C. D. Lofstrom, P. J. Lu, H. Lu, J. C. Sharpe, T. Franke and D. A. Weitz, Lab Chip, 2019, 19, 2435
DOI: 10.1039/C9LC00163H

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