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Issue 14, 2019
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Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

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Abstract

We report a microfluidic fluorescence activated cell-sorting (μFACS) device that employs traveling surface acoustic waves (TSAW) to sort cells at rates comparable to conventional jet-in-air FACS machines, with high purity and viability. The device combines inertial flow focusing and sheath flow to align and evenly space cells, improving the sorting accuracy and screening rate. We sort with an interdigital transducer (IDT) whose tapered geometry allows precise positioning of the TSAW for optimal cell sorting. We sort three different cell lines at several kHz, at cell velocities exceeding one meter per second, while maintaining both sorting purity and cell viability at around 90% simultaneously.

Graphical abstract: Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

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Publication details

The article was received on 15 Feb 2019, accepted on 04 Jun 2019 and first published on 13 Jun 2019


Article type: Paper
DOI: 10.1039/C9LC00163H
Lab Chip, 2019,19, 2435-2443

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    Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

    K. Mutafopulos, P. Spink, C. D. Lofstrom, P. J. Lu, H. Lu, J. C. Sharpe, T. Franke and D. A. Weitz, Lab Chip, 2019, 19, 2435
    DOI: 10.1039/C9LC00163H

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