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Issue 6, 2019
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Elastic reversible valves on centrifugal microfluidic platforms

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Abstract

Reversible valves on centrifugal microfluidic platforms facilitate the automation of bioanalytical assays, especially of those requiring a series of steps (such as incubation) in a single reaction chamber. In this study, we present fixed elastic reversible (FER) valves and tunable elastic reversible (TER) valves that are easy to fabricate, implement and control. In the FER valve the compression of an elastic barrier/patch against a microchamber's outlet prevents the release of liquid. The valve sealing pressure was determined by adjusting the engraving depth of the valve-seat at which the elastic patch was located, this allows to set the sealing pressure during disc fabrication. In the TER valve, the patch compression value and sealing pressure is controlled by the penetration depth of a plastic screw into the valve-seat. The ER valves prevent liquid flow until the centrifugal force overcomes their sealing pressure. Moreover, at a constant spin speed, turning the screw of a TER valve reduces its sealing pressure and opens the valve. Therefore, the TER valve allows for controlling of the liquid transfer volume at various spin speeds. The FER and TER valves' behavior is mathematically described and equations for the prediction of their operation under centrifugal forces are provided. As a point-of-care (POC) application of ER valves, we have developed a microfluidic disc with a series of TER valves and peptide microarrays for automated multiplexed detection of five different proteins from a single serum sample.

Graphical abstract: Elastic reversible valves on centrifugal microfluidic platforms

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Supplementary files

Article information


Submitted
14 Aug 2018
Accepted
11 Feb 2019
First published
11 Feb 2019

Lab Chip, 2019,19, 1090-1100
Article type
Paper

Elastic reversible valves on centrifugal microfluidic platforms

M. M. Aeinehvand, L. Weber, M. Jiménez, A. Palermo, M. Bauer, F. F. Loeffler, F. Ibrahim, F. Breitling, J. Korvink, M. Madou, D. Mager and S. O. Martínez-Chapa, Lab Chip, 2019, 19, 1090
DOI: 10.1039/C8LC00849C

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