Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 9, 2019
Previous Article Next Article

A bifunctional dermaseptin–thanatin dipeptide functionalizes the crop surface for sustainable pest management

Author affiliations

Abstract

To reduce pesticide use while preserving crop productivity, alternative pest and disease control measures are needed. We thought of an alternative way of functionalizing leaves of soybean to fight its most severe disease, Asian soybean rust (Phakopsora pachyrhizi). To do so, we produced bifunctional peptides that adhere to the soybean leaf surface and prevent the germination of P. pachyrhizi spores. In detail, amphiphilic peptides liquid chromatography peak I (LCI), thanatin (THA), tachystatin A2 (TA2), and lactoferricin B (LFB) were all fused to enhanced green fluorescent protein (eGFP). Of these fusion peptides, eGFP–LCI and eGFP–THA bound strongly and in a rainfast manner to the surface of soybean, barley, and corn leaves. eGFP–THA binding to soybean also withstood high temperature, sunlight and biotic degradation for at least 17 days. The dipeptides seem to bind mainly to the surface wax layer of leaves because eGFP–THA and eGFP–LCI did not stick to the wax-depleted cer-j59 mutant of barley or to corn leaves with their surface wax removed. A fusion of the antimicrobial peptide dermaseptin 01 and THA (DS01–THA) inhibits the germination of P. pachyrhizi spores in vitro and reduces Asian soybean rust disease in a rainfast manner. Therefore, this study reveals that bifunctional peptides can be used to functionalize the crop surface for sustainable disease management.

Graphical abstract: A bifunctional dermaseptin–thanatin dipeptide functionalizes the crop surface for sustainable pest management

Back to tab navigation

Supplementary files

Article information


Submitted
05 Feb 2019
Accepted
15 Mar 2019
First published
01 Apr 2019

This article is Open Access

Green Chem., 2019,21, 2316-2325
Article type
Paper

A bifunctional dermaseptin–thanatin dipeptide functionalizes the crop surface for sustainable pest management

P. Schwinges, S. Pariyar, F. Jakob, M. Rahimi, L. Apitius, M. Hunsche, L. Schmitt, G. Noga, C. Langenbach, U. Schwaneberg and U. Conrath, Green Chem., 2019, 21, 2316
DOI: 10.1039/C9GC00457B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements