Jump to main content
Jump to site search


PLP-dependent enzymes as important biocatalysts for the pharmaceutical, chemical and food industries: a structural and mechanistic perspective

Author affiliations

Abstract

Enzymes catalyze a plethora of chemical reactions in nature. These biocatalysts offer a variety of benefits that no other synthetic catalyst can offer: high turnover, regio- and stereoselectivity, and ability to work under mild conditions and to react in environmentally friendly manufacturing processes. However, enzymes are not normally optimally suited for industrial applications. It is important to improve their properties to enable their more efficient application in an industrial setting, while also expanding the range of potential chemical reactions that they can catalyze. In this review, we focus our analysis on structural features and mechanisms of different enzymes that contain a pyridoxal-5′-phosphate (PLP) cofactor and that have noteworthy potential as biocatalysts in a variety of industries and applications. The selected PLP enzymes are ω-transaminases, lysine decarboxylase, threonine aldolase, L-tyrosine phenol-lyase, α-amino-ε-caprolactam racemases, and cystathionine β-lyase. For each of these enzymes, the catalytic mechanism, industrial application, and the advantages and disadvantages of their application are reviewed in detail. In general, this review highlights the immense biocatalytic potential, rich chemistry and diverse set of applications that different enzymes sharing a common element (PLP) can offer.

Graphical abstract: PLP-dependent enzymes as important biocatalysts for the pharmaceutical, chemical and food industries: a structural and mechanistic perspective

Back to tab navigation

Publication details

The article was received on 19 Jun 2019, accepted on 09 Aug 2019 and first published on 09 Aug 2019


Article type: Minireview
DOI: 10.1039/C9CY01210A
Catal. Sci. Technol., 2019, Advance Article

  •   Request permissions

    PLP-dependent enzymes as important biocatalysts for the pharmaceutical, chemical and food industries: a structural and mechanistic perspective

    J. F. Rocha, A. F. Pina, S. F. Sousa and N. M. F. S. A. Cerqueira, Catal. Sci. Technol., 2019, Advance Article , DOI: 10.1039/C9CY01210A

Search articles by author

Spotlight

Advertisements