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Issue 99, 2019
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Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme

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Abstract

Complex carbapenem β-lactam antibiotics contain diverse C6 alkyl substituents constructed by cobalamin-dependent radical SAM enzymes. TokK installs the C6 isopropyl chain found in asparenomycin. Time-course analyses of TokK and its ortholog ThnK, which forms the C6 ethyl chain of thienamycin, indicate that catalysis occurs through a sequence of discrete, non-processive methyl transfers.

Graphical abstract: Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme

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Article information


Submitted
13 Sep 2019
Accepted
20 Nov 2019
First published
20 Nov 2019

Chem. Commun., 2019,55, 14934-14937
Article type
Communication

Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme

E. K. Sinner, M. S. Lichstrahl, R. Li, D. R. Marous and C. A. Townsend, Chem. Commun., 2019, 55, 14934
DOI: 10.1039/C9CC07197K

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