Issue 98, 2019

19F NMR studies on γ-butyrobetaine hydroxylase provide mechanistic insights and suggest a dual inhibition mode

Abstract

The final step in the biosynthesis of L-carnitine in humans is catalysed by the 2-oxoglutarate and ferrous iron dependent oxygenase, γ-butyrobetaine hydroxylase (BBOX). 1H and 19F NMR studies inform on the BBOX mechanism including by providing evidence for cooperativity between monomers in substrate/some inhibitor binding. The value of the 19F NMR methods is demonstrated by their use in the design of new BBOX inhibitors.

Graphical abstract: 19F NMR studies on γ-butyrobetaine hydroxylase provide mechanistic insights and suggest a dual inhibition mode

Supplementary files

Article information

Article type
Communication
Submitted
20 Aug 2019
Accepted
23 Sep 2019
First published
08 Nov 2019
This article is Open Access
Creative Commons BY license

Chem. Commun., 2019,55, 14717-14720

19F NMR studies on γ-butyrobetaine hydroxylase provide mechanistic insights and suggest a dual inhibition mode

R. K. Leśniak, A. M. Rydzik, J. J. A. G. Kamps, A. Kahn, T. D. W. Claridge and C. J. Schofield, Chem. Commun., 2019, 55, 14717 DOI: 10.1039/C9CC06466D

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