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Issue 83, 2019
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Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

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Abstract

Herein we demonstrate the first example of oligonucleotide-templated reaction (OTR) performed on paper, using lateral flow to capture and concentrate specific nucleic acid biomarkers on a test line. Quantitative analysis, using a low-cost benchtop fluorescence reader showed very high specificity down to the single nucleotide level and proved sensitive enough for amplification-free, on-chip, detection of endogenous concentrations of miR-150-5p, a recently identified predictive blood biomarker for preterm birth.

Graphical abstract: Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

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Article information


Submitted
19 Jul 2019
Accepted
16 Sep 2019
First published
17 Sep 2019

This article is Open Access

Chem. Commun., 2019,55, 12451-12454
Article type
Communication

Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

S. Pavagada, R. B. Channon, J. Y. H. Chang, S. H. Kim, D. MacIntyre, P. R. Bennett, V. Terzidou and S. Ladame, Chem. Commun., 2019, 55, 12451
DOI: 10.1039/C9CC05607F

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