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Issue 62, 2019
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4′-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

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Abstract

We designed novel 4′-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in ≈4.9-fold more efficient knockdown than unmodified siRNA.

Graphical abstract: 4′-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

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Publication details

The article was received on 29 May 2019, accepted on 05 Jul 2019 and first published on 05 Jul 2019


Article type: Communication
DOI: 10.1039/C9CC04141A
Chem. Commun., 2019,55, 9112-9115
  • Open access: Creative Commons BY license
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    4′-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

    G. N. Nawale, S. Bahadorikhalili, P. Sengupta, S. Kadekar, S. Chatterjee and O. P. Varghese, Chem. Commun., 2019, 55, 9112
    DOI: 10.1039/C9CC04141A

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