Jump to main content
Jump to site search

Issue 57, 2019
Previous Article Next Article

Spectral counterstaining in luminescence-enhanced biological Raman microscopy

Author affiliations

Abstract

Cell imaging heavily depends on fluorescent labels typically incompatible with Raman microscopy. The europium(III) complex based on dipicolinic acid (DPA) presented here is an exception from this rule. Although its luminescence bands are very narrow, their intensity is comparable to the background Raman bands. This makes it complementary to less luminous compounds referred to as Raman tags. Through several examples we show that the complex provides a morphological context in otherwise unstained cells, thus acting as a spectral-counterstaining agent.

Graphical abstract: Spectral counterstaining in luminescence-enhanced biological Raman microscopy

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Apr 2019, accepted on 20 Jun 2019 and first published on 20 Jun 2019


Article type: Communication
DOI: 10.1039/C9CC03139A
Chem. Commun., 2019,55, 8329-8332

  •   Request permissions

    Spectral counterstaining in luminescence-enhanced biological Raman microscopy

    R. Pelc, V. Mašek, V. Llopis-Torregrosa, P. Bouř and T. Wu, Chem. Commun., 2019, 55, 8329
    DOI: 10.1039/C9CC03139A

Search articles by author

Spotlight

Advertisements