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Issue 65, 2019
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Silica particles with a quercetin–R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

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Abstract

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol–peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin–peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Graphical abstract: Silica particles with a quercetin–R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

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Publication details

The article was received on 20 Mar 2019, accepted on 16 Jul 2019 and first published on 17 Jul 2019


Article type: Communication
DOI: 10.1039/C9CC02215E
Chem. Commun., 2019,55, 9649-9652
  • Open access: Creative Commons BY-NC license
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    Silica particles with a quercetin–R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

    G. Del Favero, F. Bialas, S. Grabher, A. Wittig, B. Bräuer, D. Gerthsen, C. Echalier, M. Kamalov, D. Marko and C. F. W. Becker, Chem. Commun., 2019, 55, 9649
    DOI: 10.1039/C9CC02215E

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