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Issue 29, 2019
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A microscale double labelling of GAG oligosaccharides compatible with enzymatic treatment and mass spectrometry

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Abstract

A novel double labelling of glycosaminoglycans (GAG) oligosaccharides by thia-Michael addition and deuterium incorporation at the non-reducing and reducing ends, respectively, was introduced. This was demonstrated to be both compatible with the heparin microgram scale and amenable for mass spectrometry analysis, without impairing enzymatic activities such as heparinase I and sulfatase HSulf-2.

Graphical abstract: A microscale double labelling of GAG oligosaccharides compatible with enzymatic treatment and mass spectrometry

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Publication details

The article was received on 10 Jan 2019, accepted on 05 Mar 2019 and first published on 06 Mar 2019


Article type: Communication
DOI: 10.1039/C9CC00254E
Chem. Commun., 2019,55, 4182-4185

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    A microscale double labelling of GAG oligosaccharides compatible with enzymatic treatment and mass spectrometry

    C. Przybylski, V. Bonnet and R. R. Vivès, Chem. Commun., 2019, 55, 4182
    DOI: 10.1039/C9CC00254E

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