Jump to main content
Jump to site search

Issue 6, 2019
Previous Article Next Article

In situ synthesis, stabilization and activity of protein-modified gold nanoparticles for biological applications

Author affiliations

Abstract

Herein, we demonstrate the use of lysozyme (Lys) as a model to fabricate a protein carrier system based on gold nanoparticles (AuNPs) via the Layer-by-Layer (LbL) technology. Poly(ethyleneimine) (PEI) and poly(sodium 4-styrenesulfonate) (PSS) were used as cationic and anionic polymers respectively to grow oppositely charged layers. Mild aqueous conditions were utilized to avoid protein denaturation and activity instead of organic solvents that have been used in other encapsulation systems. Two different strategies were used: (A) lysozyme acting as a reducing and stabilizing agent in the formation of AuNPs at a temperature of 45 ± 2 °C followed by only two subsequent polymeric layers deposited by LbL, and (B) citrate acting as a reducing agent prior to stabilization of the AuNPs by mercaptoundecanoic acid. Dynamic light scattering, UV-vis spectroscopy, IR spectroscopy and transmission electron microscopy were used to characterize the nanoconjugates. Furthermore, the enzymatic activity of the resulting protein/nanoparticle conjugates was evaluated using the bacteria Micrococcus lysodeikticus as a substrate.

Graphical abstract: In situ synthesis, stabilization and activity of protein-modified gold nanoparticles for biological applications

Back to tab navigation

Article information


Submitted
25 Jan 2019
Accepted
27 Mar 2019
First published
04 Apr 2019

Biomater. Sci., 2019,7, 2511-2519
Article type
Paper

In situ synthesis, stabilization and activity of protein-modified gold nanoparticles for biological applications

C. Garcia-Hernandez, A. K. Freese, M. L. Rodriguez-Mendez and A. K. Wanekaya, Biomater. Sci., 2019, 7, 2511
DOI: 10.1039/C9BM00129H

Social activity

Search articles by author

Spotlight

Advertisements