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Issue 5, 2019
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Novel colorimetric sensor based on peroxidase-like activity of chitosan-stabilized Au/Pt nanoclusters for trace lead

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Abstract

In this study we propose a new colorimetric sensor for trace lead ions in milk samples. Au/Pt nanoclusters were synthesized using chitosan as the stabilizer at room temperature. Chitosan-protected Au/Pt nanoclusters exhibit strong peroxidase-like activity, and could catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the vicinity of hydrogen peroxide (H2O2) and create a visual blue color. The detection mechanism is based on the Pb2+ ions-induced aggregation of chitosan–Au/Pt NCs that causes changes in size and impairs the enzymatic performance of Au/Pt NCs. Experiments indicated that a step-by-step increase in concentration of Pb2+ ions gradually decreased peroxidase activity and this reduction was linear. Based on the declared procedure, a colorimetric detection for Pb2+ was obtained with a detection limit (LOD) of 16 nM and a linear range from 25 nM to 1 μM. The method was considerably selective toward Pb2+ over other metal ions. Low price and simplicity support the application of sensing Pb2+ in milk samples.

Graphical abstract: Novel colorimetric sensor based on peroxidase-like activity of chitosan-stabilized Au/Pt nanoclusters for trace lead

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Supplementary files

Article information


Submitted
09 Sep 2018
Accepted
25 Dec 2018
First published
26 Dec 2018

Anal. Methods, 2019,11, 684-690
Article type
Paper

Novel colorimetric sensor based on peroxidase-like activity of chitosan-stabilized Au/Pt nanoclusters for trace lead

Z. Dehghani, M. Hosseini, J. Mohammadnejad and M. R. Ganjali, Anal. Methods, 2019, 11, 684
DOI: 10.1039/C8AY01975D

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