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Issue 2, 2019
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A diffusion NMR method for the prediction of the weight-average molecular weight of globular proteins in aqueous media of different viscosities

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Abstract

We have introduced for the first time, a non-viscosity dependent universal calibration curve (UCC) for the successful prediction of the weight-average molecular weights of globular proteins in the range of 8.5–66.2 kDa with no dependence on the solvent viscosity. Plots of log() versus log Mw showed excellent linearity (R2 > 0.99) within four different methods (LMS, TRAIn, NNLS and ITAMeD). To demonstrate the applicability of this new UCC curve, we have proposed as a proof of concept the determination of the Mw of myoglobin from an equine heart in deuterated water of four different viscosity values. The corresponding () values were fitted to the UCC to calculate their corresponding weight-average Mw that was in all the cases centered at 17 321.2 Da, with differences in the mass below 5.7% with respect to the commercial value.

Graphical abstract: A diffusion NMR method for the prediction of the weight-average molecular weight of globular proteins in aqueous media of different viscosities

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Publication details

The article was received on 16 Aug 2018, accepted on 21 Nov 2018 and first published on 12 Dec 2018


Article type: Paper
DOI: 10.1039/C8AY01817K
Anal. Methods, 2019,11, 142-147

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    A diffusion NMR method for the prediction of the weight-average molecular weight of globular proteins in aqueous media of different viscosities

    F. M. Arrabal-Campos, L. M. Aguilera-Sáez and I. Fernández, Anal. Methods, 2019, 11, 142
    DOI: 10.1039/C8AY01817K

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