Jump to main content
Jump to site search

Issue 23, 2019
Previous Article Next Article

Rapid detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP bioluminescence assay: from development to practical challenges during protocol testing in Kenya

Author affiliations

Abstract

We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS using an adenosine triphosphate (ATP) bioluminescence assay. Up to 80% GBS cells were isolated from spiked artificial urine samples with linear responses of bioluminescence signals from isolated cells at 2.3 × 102–9.1 × 105 CFU mL−1, demonstrating great promise for point-of-care detection of pathogenic bacteria in screening urine samples from pregnant women. Practical challenges during initial testing of the developed protocol with urine samples in Kenya are also described.

Graphical abstract: Rapid detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP bioluminescence assay: from development to practical challenges during protocol testing in Kenya

Back to tab navigation

Supplementary files

Article information


Submitted
13 Sep 2019
Accepted
09 Oct 2019
First published
10 Oct 2019

Analyst, 2019,144, 6889-6897
Article type
Paper

Rapid detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP bioluminescence assay: from development to practical challenges during protocol testing in Kenya

B. Ngamsom, E. A. Wandera, A. Iles, R. Kimani, F. Muregi, J. Gitaka and N. Pamme, Analyst, 2019, 144, 6889
DOI: 10.1039/C9AN01808E

Social activity

Search articles by author

Spotlight

Advertisements