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Issue 18, 2019
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Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

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Abstract

Complicated DNA molecular behaviors exist during translocation into a nanopore because their large and coiled structure needs to unwind. In this work, we investigated DNA translocation dynamics through a 200 nm pore using a fast photon counting system (FPCS). We found that the dwell time of the DNA molecules depends on the inverse of voltage (τV−1.02) with a large constant term (∼1 ms). In other words, spherical fluorescence bead translocation involves electrophoresis as well as other additional factors. Our theoretical calculation suggested that one additional factor is electro-osmotic trapping associated with the instantaneous Brownian motion before and after translocation. Furthermore, compressed DNA molecular conformation was seen as a result of the increase of peak photon counts and the decrease of electrophoretic mobility with voltage. Our experiments showed that the polymers at the vicinity of a nanopore can be trapped and compressed, which is necessary to understand how to control the polymer translocation into a nanopore.

Graphical abstract: Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

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Supplementary files

Article information


Submitted
04 Jul 2019
Accepted
17 Aug 2019
First published
19 Aug 2019

Analyst, 2019,144, 5381-5388
Article type
Communication

Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

H. Yamazaki, T. Mizuguchi, K. Esashika and T. Saiki, Analyst, 2019, 144, 5381
DOI: 10.1039/C9AN01253B

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