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Issue 8, 2019
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A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

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Abstract

Hydrogen peroxide (H2O2), as a major component of reactive oxygen species (ROS), plays an important role in normal physiological processes. A H2O2 burst also occurs in the ischemia/reperfusion (I/R) process and causes a series of physiological and pathological injuries. Therefore, it is important to determine concentration fluctuations of H2O2. Here we develop a ratiometric fluorescent probe, Cy-ArB, which shows high selectivity and sensitivity toward H2O2. The fluorescence response of the probe is triggered by the reaction of borate esters with H2O2, and this process releases a near-infrared heptamethine cyanine fluorophore which has the ability of mitochondrial tracing. Hence, the probe can be used for real-time monitoring of H2O2 fluctuations in the mitochondrial respiration chain. Finally, we explore the fluctuations of H2O2 in cells and in vivo during the I/R process using the probe Cy-ArB. The results of our experiments prove that our probe is a potential candidate for clinical surgery pre-evaluation.

Graphical abstract: A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

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Supplementary files

Article information


Submitted
03 Feb 2019
Accepted
06 Mar 2019
First published
07 Mar 2019

Analyst, 2019,144, 2556-2564
Article type
Paper

A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

R. Xu, Y. Wang, H. You, L. Zhang, Y. Wang and L. Chen, Analyst, 2019, 144, 2556
DOI: 10.1039/C9AN00243J

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