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Issue 8, 2019
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Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

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Abstract

Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. Numerous methods have been developed for related analyses, including the detection of O2 consumption, trans-membrane potential, and ATP production. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Raman imaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viability tests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.

Graphical abstract: Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

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Supplementary files

Article information


Submitted
15 Nov 2018
Accepted
14 Feb 2019
First published
15 Feb 2019

Analyst, 2019,144, 2531-2540
Article type
Paper

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

T. Morimoto, L. Chiu, H. Kanda, H. Kawagoe, T. Ozawa, M. Nakamura, K. Nishida, K. Fujita and T. Fujikado, Analyst, 2019, 144, 2531
DOI: 10.1039/C8AN02213E

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