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Issue 5, 2019
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Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

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Abstract

Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid–liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.

Graphical abstract: Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

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Supplementary files

Article information


Submitted
04 Nov 2018
Accepted
28 Dec 2018
First published
04 Jan 2019

Analyst, 2019,144, 1600-1607
Article type
Paper

Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

L. Morelli, F. A. Centorbi, O. Ilchenko, C. B. Jendresen, D. Demarchi, A. T. Nielsen, K. Zór and A. Boisen, Analyst, 2019, 144, 1600
DOI: 10.1039/C8AN02128G

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