Jump to main content
Jump to site search


Oxidative Stress-Mediated Apoptotic Cell Death Induced by Camphor in sod1-Deficient Schizosaccharomyces pombe

Abstract

Camphor is one of the monoterpenes widely used in cosmetics, pharmacy and food industry. In this study, we aimed to assess oxidative, cytotoxic and apoptotic effects of camphor in the fission yeast (Schizosaccharomyces pombe), which is a promising unicellular model organism in mechanistic toxicology and cell biology. Since Sod1 is the main radical scavenger in the cell, we used sod1 mutants to understand whether camphor-induced ROS accumulation caused higher cytotoxicity and apoptosis. Camphor exposure (0-2000 mg/L) caused significant cytotoxicity in yeast, particularly in sod1 cells. DCFDA (2,7-dichlorodihydrofluorescein diacetate) fluorescence and NBT (p-nitro-blue tetrazolium chloride) reduction increased (at least 2.5-3-fold in sod1 cells) in correlation with camphor concentrations (800-1200 mg/L), showing higher ROS levels and oxidative stress. Moreover, cells, stained with acridine orange/ethidium bromide, showed apoptotic morphology with nuclear fragmentation and condensation. DAPI (4’,6-diamidino-2-phenylindole) staining was used to validate apoptotic nuclear morphology. Dramatically increased mitochondrial impairment, which was higher in sod1 cells than in wild type cells, was shown by Rhodamine 123 staining. In conclusion, camphor-induced excessive ROS production, which could not be prevented significantly in sod1 mutants, caused dramatic increase in mortality rates due to intrinsic apoptosis revealed by mitochondrial impairment and apoptotic nuclear morphology. The potential effects of camphor on apoptotic cell death and accounting mechanisms were clarified in unicellular eukaryotic model, S. pombe.

Back to tab navigation

Publication details

The article was received on 13 Oct 2018, accepted on 26 Nov 2018 and first published on 27 Nov 2018


Article type: Paper
DOI: 10.1039/C8TX00279G
Citation: Toxicol. Res., 2018, Accepted Manuscript
  •   Request permissions

    Oxidative Stress-Mediated Apoptotic Cell Death Induced by Camphor in sod1-Deficient Schizosaccharomyces pombe

    H. H. Agus, C. O. Sengoz and S. Yilmaz, Toxicol. Res., 2018, Accepted Manuscript , DOI: 10.1039/C8TX00279G

Search articles by author

Spotlight

Advertisements