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Arsenic aggravated reproductive toxicity in male rats exposed to lead during perinatal period

Abstract

The aim of this study was to assess the reproductive toxic effects of arsenic in adult Wistar rats exposed to lead during perinatal period. The pregnant rats were allowed ad libitum access to tap water containing 819 mg of lead (Pb)/L or without Pb from conception till weaning. Litter size, survival rate and developmental milestones of pups delivered by Pb exposed dams were comparable to control rats. Conversely, pups exposed to Pb during perinatal period exhibited significant delay in cliff avoidance, negative geotaxis, surface righting reflex, ascending wire mesh and testis descent. Control and perinatal Pb-exposed male rats were maintained on tap water containing 2.3 mg of arsenite (As)/L or without arsenite from pubertal period (post-natal day 55) to adulthood (post-natal 115) and assessed for reproductive end points. Results revealed that 1) relative weights of testis, epididymis, seminiferous tubules and ventral prostate; 2) daily sperm production; 3) epididymal sperm density, numbers of motile-, viable-, and HOS tail swelled sperm declined significantly in rats exposed to either Pb or As. The activity levels of testicular 3β- and 17β-hydroxysteroid dehydrogenases were also significantly decreased in experimental rats. Significant elevation in levels of reactive oxygen species and lipid peroxidation in association with reduced activities of antioxidant enzymes in the testis and different epididymal regions were recorded in experimental rats. In the fertility study, although each male in control and experimental groups produced a copulatory plug and impregnated a female, the mean conception time significantly increased in experimental groups. The mean number of implantations decreased significantly in females mated with experimental males. Moreover, the results of the present study also indicate that reproductive alterations were more deteriorated in Pb-exposed rats treated with arsenic when compared to individual exposures. In conclusion, the data clearly suggests that reproductive toxicity in male rats exposed to Pb during perinatal period is exacerbated by As treatment at pubertal period.

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Publication details

The article was received on 30 May 2018, accepted on 08 Aug 2018 and first published on 09 Aug 2018


Article type: Paper
DOI: 10.1039/C8TX00146D
Citation: Toxicol. Res., 2018, Accepted Manuscript
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    Arsenic aggravated reproductive toxicity in male rats exposed to lead during perinatal period

    S. S. A.K, P. K. Reddy, P. G. Girish, S. Ch and S. P. Reddy, Toxicol. Res., 2018, Accepted Manuscript , DOI: 10.1039/C8TX00146D

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