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In vitro modulation of mercury-induced rat liver mitochondria dysfunction

Abstract

Mercury (Hg) is a toxic environmental pollutant that exerts its cytotoxic effects as cations by targeting mitochondria. In our work, we determined different mitochondrial toxicity factors using specific substrates and inhibitors following addition of Hg2+ to the mitochondria isolated from Wistar rat liver in vitro. We found that Hg2+ induced marked changes in mitochondrial ultrastructure and accompanied by mitochondrial swelling, mitochondrial membrane potential collapse, mitochondrial membrane fluidity increase and Cytochrome c release. Additionally, the effects of Hg2+ on heat production of mitochondria were investigated with microcalorimetry; simultaneously, the effects on mitochondrial respiration were determined by clark oxygen-electric methods. Microcalorimetry can provide detailed kinetic and thermodynamic information which demonstrated that Hg2+ had some biotoxicity effect on mitochondria. The inhibition of energy metabolic activities suggested high concentrations of Hg2+ could induce mitochondrial ATP depletion under MPT and mitochondrial respiration inhibition. These results help us learn more about the toxicity of Hg2+ at the subcellular level.

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Publication details

The article was received on 23 Feb 2018, accepted on 09 Jul 2018 and first published on 10 Jul 2018


Article type: Paper
DOI: 10.1039/C8TX00060C
Citation: Toxicol. Res., 2018, Accepted Manuscript
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    In vitro modulation of mercury-induced rat liver mitochondria dysfunction

    L. Ma, K. Bi, Y. Fan, Z. Jiang, X. Zhang, J. Zhang, J. Zhao, F. Jiang and J. Dong, Toxicol. Res., 2018, Accepted Manuscript , DOI: 10.1039/C8TX00060C

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