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In vitro antioxidant efficacy and the therapeutic potential of Wendlandia heynei (Schult.) Santapau & Merchant against bisphenol A induced hepatotoxicity in rat

Abstract

The aim of present study was to access the antioxidant and ameliorative efficacy of Wendlandia heynei stem bark’s crude methanol extract (WHBM) against the bisphenol A (BPA) induced hepatotoxicity in rat. WHBM and its derived fractions exhibited promising activity for scavenging of DPPH, hydroxyl and nitrite radicals, iron chelation and for inhibition of β-carotene oxidation. Administration of BPA to Sprague Dawley rat (25 mg/kg) for 28 days resulted in elevated (p < 0.01) level of aspartate transaminase, alanine transaminase, alkaline phosphatase, and globulin while decrease (p < 0.01) the level of total protein and albumin in serum of rat. In hepatic samples level of catalase, peroxidase, superoxide dismutase and glutathione-S-transferase, reduced glutathione content was decreased (p < 0.05) whereas thiobarbituric acid reactive substances, hydrogen peroxide and nitrite content increased (p < 0.05) with BPA treatment to rat. Administration of WHBM to BPA intoxicated rat restored the altered level of these parameters towards the control animals. Histopathological alterations of hepatic tissues induced with BPA were restored with WHBM co-treatment to rat. HPLC-DAD analysis ensured the occurrence of rutin, catechin and caffeic acid in WHBM and WHBE. Results of this study suggested that presence of phenolics and flavonoids in W. heynei bark might exhibited antioxidant potential during in vitro and in vivo studies and hence can be used as a therapeutic agent against oxidative stress associated diseases.

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Publication details

The article was received on 05 Dec 2017, accepted on 02 Aug 2018 and first published on 08 Aug 2018


Article type: Paper
DOI: 10.1039/C7TX00322F
Citation: Toxicol. Res., 2018, Accepted Manuscript
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    In vitro antioxidant efficacy and the therapeutic potential of Wendlandia heynei (Schult.) Santapau & Merchant against bisphenol A induced hepatotoxicity in rat

    S. Maryam, M.R. Khan, S. A. Shah, Z. Zahra, M. Majid, M. Sajid and S. Ali, Toxicol. Res., 2018, Accepted Manuscript , DOI: 10.1039/C7TX00322F

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