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Multifunctional luminescent immuno-magnetic nanoparticles: toward fast, efficient, cell-friendly capture and recovery of circulating tumor cells

Abstract

Highly efficient isolation and recovery of viable circulating tumor cells (CTCs) from patients’ blood is an important precondition to address the current dilemma on insufficient CTCs study, which can promote the development of individualized antitumor therapy. Herein, a cell-friendly CTC isolation and recovery nanoplatform with luminescent labelling was established using the layer-by-layer (LbL) assembly technique and the stimulus cellular-release strategy. Specially, the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody was introduced with a disulfide bond-containing linker for further bio-friendly recovery of CTCs. And quantum dots (QDs) were deposited onto fast magnet-responsive Fe3O4 nanoparticles through the facile LbL assembly method to monitor the capture and recovery process real-timely. The obtained PEGlyated immuno-magnetic nanospheres (PIMNs) can be all magnetically collected within 2 min. Capture efficiency above 90% can be achieved from blood samples with 5-200 CTCs per mL via only 1-2 min incubation. Nearly all PIMNs on the surface of CTCs were detached after 15 min of glutathione (GSH) treatment with the disappearance of QDs signals. Recovered CTCs could be directly used for culture (cell viability, ~98%), and their invasiveness and migration characteristics remained unchanged. Furthermore, the PIMNs were successfully applied to isolate CTCs in cancer patients’ peripheral blood samples, an average of 8.6±5.8 CTCs per mL was detected. Results above suggested that PIMNs may serve as a powerful nanoplatform for CTCs screening, isolation and recovery.

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Publication details

The article was received on 13 Oct 2018, accepted on 23 Nov 2018 and first published on 26 Nov 2018


Article type: Paper
DOI: 10.1039/C8TB02701C
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Multifunctional luminescent immuno-magnetic nanoparticles: toward fast, efficient, cell-friendly capture and recovery of circulating tumor cells

    X. Zhou, B. Luo, K. Kang, S. Ma, X. Sun, F. Lan, Q. Yi and Y. Wu, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB02701C

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