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Synergic TiO2 photocatalysis and guanine photoreduction for silver deposition amplification: An ultrasensitive and high-throughput visualized colorimetric analysis strategy for Anthrax DNAs in blood using wettable microwells array

Abstract

An ultrasensitive and high-throughput visualized colorimetric method has been initially developed with wettable microwells array for probing guanine bases-containing Anthrax DNAs in blood based on the silver deposition amplified by the synergic TiO2 photocatalysis and guanine photoreduction under visible light. Hydrophilic microwells were first created on the hydrophobic slides to yield the wettable microwells array, on which photocatalytic titanium dioxide (TiO2) nanoparticles were deposited with dopamine (DA) to yield TiO2@DA for anchoring single strand DNA (ssDNA) capture probes without guanine bases. After the hybridization of targeting Anthrax DNAs, exonuclease I (Exo I) was introduced into the microwells to selectively digest the ssDNA probes unhybridized. The silver deposition was further conducted by the synergic photocatalysis of TiO2@DA and photoreduction of guanine bases of Anthrax DNAs, thus achieving the amplified silver signals for the visualized colorimetric assays. Moreover, benefitting from the wettability feature of hydrophilic-hydrophobic interfaces of microwells array so fabricated, DNA analytes could be accumulated from the sample droplets through the condensing enrichment process to realize the ultrasensitive detections, in addition to circumventing any crossover contaminants between the sample droplets. The developed visualized colorimetric method with microwells array was subsequently applied for probing Anthrax DNAs in blood with the levels down to 1.0 fM. Single-base and double-base mutations DNAs could also be identified accurately. Importantly, such a biosensing design route of wettable microwells array, in combination with the photocatalytic silver deposition and specific Exo I-catalytic probe digestion, may promise the extensive applications for the high-throughput, ultrasensitive, and selective detections of guanine-containing DNA targets with ultra-trace levels in the complicated samples like blood.

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Publication details

The article was received on 23 Sep 2018, accepted on 08 Oct 2018 and first published on 11 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB02500B
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Synergic TiO2 photocatalysis and guanine photoreduction for silver deposition amplification: An ultrasensitive and high-throughput visualized colorimetric analysis strategy for Anthrax DNAs in blood using wettable microwells array

    H. Liu, L. Feng, Y. Cai, Y. Hua, M. Liu, M. Yin, S. Li, X. Lv, J. Wen and H. Wang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB02500B

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