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Issue 48, 2018
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Pyridine boronic acid-polyoxometalate based porous hybrid for efficient depletion of high abundant glycoproteins in plasma

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Abstract

A porous hybrid, namely PW12@TiO2–Si(Et)Si/Pba, is fabricated by the modification of PW12@TiO2–Si(Et)Si with pyridine boronic acid via a solvothermal method. The covalent interactions between the boronic acid group of the hybrid and the glycosylated site of proteins provide the as-prepared hybrid with favorable adsorption performance towards glycoproteins. The adsorption behaviors of glycoproteins onto the hybrid fit well with the Langmuir model, and the adsorption capacities for glycoprotein IgG and Trf are high, up to 348.5 mg g−1 and 262.6 mg g−1, respectively. Thus, a protocol for the depletion of high abundant glycoproteins from human plasma is proposed. The percentage contents of Trf, IgG, haptoglobinis and IgM are reduced from 7.38%, 5.5%, 3.01% and 1.02% to 0.951%, 4.7%, 0.126% and 0.76%, respectively. 85 low abundant proteins are identified from the raw plasma after the depletion of the high abundance proteins, demonstrating the potential of PW12@TiO2–Si(Et)Si/Pba hybrid in proteomic studies.

Graphical abstract: Pyridine boronic acid-polyoxometalate based porous hybrid for efficient depletion of high abundant glycoproteins in plasma

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Publication details

The article was received on 28 Aug 2018, accepted on 13 Nov 2018 and first published on 14 Nov 2018


Article type: Paper
DOI: 10.1039/C8TB02265H
Citation: J. Mater. Chem. B, 2018,6, 8196-8203
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    Pyridine boronic acid-polyoxometalate based porous hybrid for efficient depletion of high abundant glycoproteins in plasma

    X. Hu, Q. Chen, D. Zhang, X. Chen and J. Wang, J. Mater. Chem. B, 2018, 6, 8196
    DOI: 10.1039/C8TB02265H

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