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Improving the adsorption capacity for ovalbumin by functional modification of aminated mesoporous silica nanoparticles with tryptophan

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Abstract

Tryptophan (Trp) modified aminated mesoporous silica nanoparticles (AMSNs), shortened to Trp-AMSNs, are prepared via covalent binding. The obtained Trp-AMSNs exhibit a uniform size of ca. 83 nm, a mesopore diameter of ca. 2.6 nm, along with a pore volume of 0.439 cm3 g−1. It is demonstrated that Trp-AMSNs selectively adsorb ovalbumin (Ova) from complex biological matrices. At pH 5.0, 1.0 mg of Trp-AMSNs produces an adsorption efficiency of 96% for 100 mg L−1 Ova in 1.0 mL of solution. An adsorption capacity of 1240.3 mg g−1 is derived for Ova, which is much improved with respect to that of the native AMSNs. The retained Ova could be readily recovered by a sodium dodecyl sulfate (SDS) solution (0.5%, m/v), providing a recovery of 71.2%. Trp-AMSNs are further applied for the isolation of Ova from a protein mixture (with a molar ratio of ovalbumin/lysozyme of 1 : 10) and an egg-white sample. High-purity Ova is obtained, as demonstrated by SDS-PAGE assay results.

Graphical abstract: Improving the adsorption capacity for ovalbumin by functional modification of aminated mesoporous silica nanoparticles with tryptophan

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Publication details

The article was received on 23 Aug 2018, accepted on 16 Oct 2018 and first published on 17 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB02221F
Citation: J. Mater. Chem. B, 2018, Advance Article
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    Improving the adsorption capacity for ovalbumin by functional modification of aminated mesoporous silica nanoparticles with tryptophan

    M. Qiao, X. Liu, J. Song, T. Yang, M. Chen and J. Wang, J. Mater. Chem. B, 2018, Advance Article , DOI: 10.1039/C8TB02221F

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