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Co3O4 nanocrystals as an efficient catalase mimic for colorimetric detection of glutathione

Abstract

Nanomaterial-based artificial enzymes (nanozymes), as an emerging generation of artificial enzymes, has received extensive attention in recent years owing to its striking merits. In this study, the obtained Co3O4 nanocrystals exhibited catalase-like activity, which could catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H2O2 and a clear absorption peak at 652 nm can be observed. It was verified that the catalytic activity for the oxidation of TMB originated from the oxygen, which is generated by Co3O4 catalyzed H2O2 decomposition. With the addition of glutathione (GSH), the catalytic ability of Co3O4 nanocrystals was inhibited. And the changes of absorbance at 652 nm could be utilized to quantify the concentration of GSH. Under the optimized conditions, the proposed assay showed good linear relationships and low detection limit towards GSH. Moreover, the Co3O4 nanocrystals had excellent stability and can keep the catalytic activity for a long time. Thus, a simple, sensitive and selective nanozyme-based biosensor was developed for colorimetric detection of GSH.

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Publication details

The article was received on 27 Jul 2018, accepted on 14 Sep 2018 and first published on 15 Sep 2018


Article type: Paper
DOI: 10.1039/C8TB01948G
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Co3O4 nanocrystals as an efficient catalase mimic for colorimetric detection of glutathione

    W. Li, J. Wang, J. Zhu and Y. Zheng, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01948G

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