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Co3O4 nanocrystals as an efficient catalase mimic for the colorimetric detection of glutathione

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Abstract

Nanomaterial-based artificial enzymes (nanozymes), as an emerging generation of artificial enzymes, have received extensive attention in recent years owing to their striking merits. In this study, the obtained Co3O4 nanocrystals exhibited catalase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2, and a clear absorption peak at 652 nm can be observed. It was verified that the catalytic activity for the oxidation of TMB originated from the oxygen, which is generated by Co3O4 catalyzed H2O2 decomposition. Upon addition of glutathione (GSH), the catalytic ability of the Co3O4 nanocrystals was inhibited. And, the changes of absorbance at 652 nm could be utilized to quantify the concentration of GSH. Under the optimized conditions, the proposed assay showed good linear relationships and a low detection limit towards GSH. Moreover, the Co3O4 nanocrystals had excellent stability and can maintain the catalytic activity for a long time. Thus, a simple, sensitive and selective nanozyme-based biosensor was developed for the colorimetric detection of GSH.

Graphical abstract: Co3O4 nanocrystals as an efficient catalase mimic for the colorimetric detection of glutathione

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Publication details

The article was received on 27 Jul 2018, accepted on 14 Sep 2018 and first published on 15 Sep 2018


Article type: Paper
DOI: 10.1039/C8TB01948G
Citation: J. Mater. Chem. B, 2018, Advance Article
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    Co3O4 nanocrystals as an efficient catalase mimic for the colorimetric detection of glutathione

    W. Li, J. Wang, J. Zhu and Y. Zheng, J. Mater. Chem. B, 2018, Advance Article , DOI: 10.1039/C8TB01948G

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