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Issue 41, 2018
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Porous nanosheet wrapping for live imaging of suspension cells

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Abstract

In the field of cell imaging, it is still a practical challenge to obtain the high quality live imaging of suspension cells, mainly due to undesirable cell movement in the imaging field during observation. This study describes a porous nanosheet wrapping method to noninvasively immobilize suspension cells for their live imaging. Perforated nanopores are fabricated on a nanosheet to enable the addition of external chemicals to cells, ranging from small molecules to macromolecules. Through several case studies, such as the live imaging of membrane staining of liposomes, transferrin endocytosis of B cells, and activation of platelets, it is verified that the confined space made by the nanosheet could provide a hydrodynamically stable environment for suspension cells, even if an aqueous stimulus is added through the nanopores in a static or a flowing condition. With this method, the live imaging of the whole activation process on a specific suspension cell in the imaging field is achieved, which is not feasible with the existing cell immobilization methods. This study suggests that the method of porous nanosheet wrapping will facilitate the visualization of the dynamic functions of suspension cells.

Graphical abstract: Porous nanosheet wrapping for live imaging of suspension cells

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Article information


Submitted
27 Jul 2018
Accepted
12 Sep 2018
First published
13 Sep 2018

This article is Open Access

J. Mater. Chem. B, 2018,6, 6622-6628
Article type
Paper

Porous nanosheet wrapping for live imaging of suspension cells

H. Zhang, T. Aoki, K. Hatano, K. Kabayama, M. Nakagawa, K. Fukase and Y. Okamura, J. Mater. Chem. B, 2018, 6, 6622 DOI: 10.1039/C8TB01943F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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