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Selective Tracking of Ovarian-Cancer-Specific γ-Glutamyltranspeptidase by a Ratiometric Two-Photon Fluorescent Probe

Abstract

Real-time tracking of GGT enzymatic activity in human ovarian cancers cells is a reliable way for accurate prediction of cancer diagnosis and management. Here, we reported the two-photon ratiometric tracking of GGT activity in cancer cells based on a probe with Förster resonance energy transfer switchable property. In the absence of GGT, the designed probe showed two well-resolved emission bands at 461 and 610 nm, corresponding to the 7-hydroxycoumarin donor and BODIPY acceptor, respectively. In contrast, GGT catalyzed cascade reactions including cleavage of the γ-glutamyl group and subsequent aromatic hydrocarbon transfer from S to N atom enlarged the distance between the two chromophores, thus decreasing the FRET efficiency with recovering the donor fluorescence at 461 nm. By exploiting this enzyme-triggered ratiometric measurement, successful differentiation of ovarian cancer cells from normal cells with this probe was realized by two-photon fluorescence confocal microscopy.

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Publication details

The article was received on 03 Jul 2018, accepted on 04 Oct 2018 and first published on 04 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB01735B
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Selective Tracking of Ovarian-Cancer-Specific γ-Glutamyltranspeptidase by a Ratiometric Two-Photon Fluorescent Probe

    C. Zhao, B. Shi, Z. zhang, Q. Jin, Z. Wang, J. Tang, G. Xu, T. Zhu, X. Gong and X. Tang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01735B

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