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Selective tracking of ovarian-cancer-specific γ-glutamyltranspeptidase using a ratiometric two-photon fluorescent probe

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Abstract

Real-time tracking of GGT enzymatic activity in human ovarian cancer cells is a reliable method for accurate prediction of cancer diagnosis and management. Here, we report the two-photon ratiometric tracking of GGT activity in cancer cells based on a probe with switchable Förster resonance energy transfer properties. In the absence of GGT, the designed probe showed two well-resolved emission bands at 461 and 610 nm, corresponding to the 7-hydroxycoumarin donor and BODIPY acceptor, respectively. In contrast, GGT catalyzed cascade reactions including cleavage of the γ-glutamyl group and subsequent aromatic hydrocarbon transfer from the S to N atom increased the distance between the two chromophores, thus decreasing the FRET efficiency, with the recovery of the donor fluorescence at 461 nm. By exploiting this enzyme-triggered ratiometric measurement, successful differentiation of ovarian cancer cells from normal cells with this probe was realized by two-photon fluorescence confocal microscopy.

Graphical abstract: Selective tracking of ovarian-cancer-specific γ-glutamyltranspeptidase using a ratiometric two-photon fluorescent probe

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Publication details

The article was received on 03 Jul 2018, accepted on 04 Oct 2018 and first published on 04 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB01735B
Citation: J. Mater. Chem. B, 2018, Advance Article
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    Selective tracking of ovarian-cancer-specific γ-glutamyltranspeptidase using a ratiometric two-photon fluorescent probe

    B. Shi, Z. Zhang, Q. Jin, Z. Wang, J. Tang, G. Xu, T. Zhu, X. Gong, X. Tang and C. Zhao, J. Mater. Chem. B, 2018, Advance Article , DOI: 10.1039/C8TB01735B

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