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Facile synthesis of near-infrared emitting dBSA-templated Cu nanoclusters for sensitive detection of heparin

Abstract

Heparin is the most widely studied glycosaminoglycans. It plays an important role in regulating several normal physiological and pathological processes including inhibition of thrombocytopenia, lipid regulation, metabolism and electrostatic attraction with various proteins. So it is very crucial that developing selective and sensitive assays for monitoring heparin levels in biological system. In the present study, we designed a facile synthesis method to prepare near-infrared emitting denatured bovine serum album templated copper nanoclusters (dBSA-Cu NCs) for the trace detection of heparin. In aqueous solution, the bovine serum album (BSA) was denatured by heated at a temperature of 70 °C for the preparation of the dBSA, which was employed as the template for fabricating the dBSA-Cu NCs. Then Cu2+ ions were directly reduced into dBSA-Cu NCs by hydrazine hydrate at room temperature. The synthetic process was very simple for controlling due to the lack of any complicated procedure, such as heating or adjusting pH. In addition, the fluorescence intensity of dBSA-Cu NCs at 642 nm can be obviously quenched by heparin samples. So the dBSA-Cu NCs can be used as a probe for the trace detection of heparin with a very low limit of detection (LOD) of 0.26 ng/mL in a liner range from 1.25 ng/mL to 250 ng/mL. Furthermore, the as-developed dBSA-Cu NCs were also successfully applied to determine heparin in human plasma samples with quantitative spike recoveries from 95% to 104%.

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Publication details

The article was received on 02 Jul 2018, accepted on 30 Jul 2018 and first published on 02 Aug 2018


Article type: Paper
DOI: 10.1039/C8TB01733F
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Facile synthesis of near-infrared emitting dBSA-templated Cu nanoclusters for sensitive detection of heparin

    X. Wu, F. Zhang and Y. Li, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01733F

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