Issue 39, 2018

A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI

Abstract

Polyethylene glycol (PEG) is a promising candidate for protein resistance and preserving protein function in biomedical applications. In this study, a PEG-based bifunctional platform with antifouling for plasma proteins and high sensitivity for biomolecules was designed. Long PEG chains (PEG24) were used to install functional biomolecules, and short PEG chains (PEG4) served as a protective layer to backfill the surface and suppress nonspecific protein adsorption. Quartz crystal microbalance with dissipation (QCM-D) and dual polarization interferometry (DPI) were combined to investigate the dynamic process of PEG4 backfilling and the recognition capacity of biomolecules with different ratios of PEG4 and PEG24 in real time. The amount of PEG4 chain backfilling affected the flexibility of PEG24 and exposed sites. The recognition capacity was improved by increasing the ratios of PEG4 to PEG24. Therefore, when the feeding ratio of PEG4 to PEG24 was 9 : 1, a highly efficient and sensitive platform was constructed for immobilization of antibodies and recognition of antigens either in pure PBS or in a complex biological environment.

Graphical abstract: A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI

Article information

Article type
Paper
Submitted
10 Jun 2018
Accepted
03 Sep 2018
First published
21 Sep 2018

J. Mater. Chem. B, 2018,6, 6217-6224

A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI

Y. Du, J. Jin and W. Jiang, J. Mater. Chem. B, 2018, 6, 6217 DOI: 10.1039/C8TB01526K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements