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In vitro and in vivo toxicity evaluation of halloysite nanotubes


Because of their outstanding properties, increasing numbers of researches and emerging of applications in manufactured products are being in progress for halloysite nanotubes (HNTs). Therefore, the impact of HNTs on the environment and human health should be taken into consideration. In order to clearly show the cell uptake of HNTs and the biodistribution of HNTs in zebrafish, HNTs are labeled with fluorescein isothiocyanate (FITC-HNTs). The cytotoxicity assays showed the cell viabilities of human umbilical vein endothelial cells (HUVECs) and human breast adenocarcinoma (MCF-7) cells were above 60% after treated with different concentration of HNTs (2.5-200 μg/mL) for 72 h. Confocal laser scanning microscope (CLSM) results showed the uptake of HNTs by HUVECs and MCF-7 cells. The in vivo toxicity of HNTs was then investigated in early developing zebrafish embryos. The percent survival of zebrafish embryos and larvae showed no significant changes at different developmental stages (24, 48, 72, 96, and 120 hpf) when treated with various concentrations of HNTs (0.25~10 mg/mL). Besides, HNTs could promote the hatchability of zebrafish embryos and could not affect the morphological development of zebrafish at the concentration of ≤ 25 mg/mL. HNTs also could be ingested by zebrafish larvae and majorly accumulated in the gastrointestinal tract. The fluorescence intensity of FITC-HNTs was decreased gradually with time, which suggested that HNTs could be excreted by zebrafish larvae through the gastrointestinal metabolism. Therefore, it can be concluded that HNTs are relatively biocompatible nanomaterials which can be utilized in many fields.

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Publication details

The article was received on 26 May 2018, accepted on 10 Jul 2018 and first published on 10 Jul 2018

Article type: Paper
DOI: 10.1039/C8TB01382A
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    In vitro and in vivo toxicity evaluation of halloysite nanotubes

    Z. Long, Y. Wu, H. Gao, J. Zhang, X. Ou, R. He and M. Liu, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01382A

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