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Complexes of Magnetic Nanospheres with Amphiprotic Polymer-Zn Systems for Selective Isolation of Lactoferrin

Abstract

An amphiprotic polymer-Zn complex magnetic nanospheres termed as Fe3O4@PCL-CMC-Zn is designed and prepared via a stepwise synthetic strategy. Hydrophobic polycaprolactone (PCL) is firstly coated onto the magnetic Fe3O4 nanospheres, then hydrophilic carboxymethylcellulose(CMC) is grafted onto the hydrophobic PCL blocks via esterification reaction, followed by the final chelating with Zn2+ ion. The homogeneous core-shell structure and fasten amphiprotic polymer layer provides the as-prepared Fe3O4@PCL-CMC-Zn magnetic nanospheres improved protein binding behaviors, and the chelated Zn2+ offers it with favorite adsorption selectivity towards apo-lactoferrin. The adsorption capacity of apo-lactoferrin is high up to 615.3 mg g-1. The exploiting of FeCl3 as stripping reagent not only provides efficient recovery of the adsorbed apo-lactoferrin, i.e., a recovery of 83.2%, but also achieves the restoring of lactoferrin structure. Fe3O4@PCL-CMC-Zn magnetic nanospheres are then employed as sorbent for the selective isolation of lactoferrin from human colostrum sample, obtaining high-purity lactoferrin as demonstrated by SDS-PAGE and Q-TOF LC-MS assays.

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Publication details

The article was received on 22 May 2018, accepted on 07 Aug 2018 and first published on 07 Aug 2018


Article type: Paper
DOI: 10.1039/C8TB01341A
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Complexes of Magnetic Nanospheres with Amphiprotic Polymer-Zn Systems for Selective Isolation of Lactoferrin

    Z. Guo, D. Zhang, S. Song, Y. Shu, X. Chen and J. Wang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01341A

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