Jump to main content
Jump to site search

Issue 35, 2018
Previous Article Next Article

Complexes of magnetic nanospheres with amphiprotic polymer–Zn systems for the selective isolation of lactoferrin

Author affiliations

Abstract

Amphiprotic polymer–Zn complex magnetic nanospheres, termed Fe3O4@PCL–CMC–Zn, are designed and prepared via a step-wise synthetic strategy. Hydrophobic polycaprolactone (PCL) is firstly coated onto the magnetic Fe3O4 nanospheres, and then hydrophilic carboxymethylcellulose (CMC) is grafted onto the hydrophobic PCL blocks via an esterification reaction, followed by finally chelating with Zn2+ ions. The homogeneous core–shell structure and fastened amphiprotic polymer layer provide the as-prepared Fe3O4@PCL–CMC–Zn magnetic nanospheres with improved protein binding behavior, and the chelated Zn2+ offers the nanospheres favorable adsorption selectivity towards apo-lactoferrin. The adsorption capacity of apo-lactoferrin is high, up to 615.3 mg g−1. The exploitation of FeCl3 as a stripping reagent not only provides efficient recovery of the adsorbed apo-lactoferrin, i.e. a recovery of 83.2%, but also achieves the restoration of the lactoferrin structure. The Fe3O4@PCL–CMC–Zn magnetic nanospheres are then employed as a sorbent for the selective isolation of lactoferrin from human colostrum samples, obtaining high-purity lactoferrin as demonstrated by SDS-PAGE and Q-TOF LC-MS assays.

Graphical abstract: Complexes of magnetic nanospheres with amphiprotic polymer–Zn systems for the selective isolation of lactoferrin

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 May 2018, accepted on 07 Aug 2018 and first published on 07 Aug 2018


Article type: Paper
DOI: 10.1039/C8TB01341A
Citation: J. Mater. Chem. B, 2018,6, 5596-5603
  •   Request permissions

    Complexes of magnetic nanospheres with amphiprotic polymer–Zn systems for the selective isolation of lactoferrin

    Z. Guo, D. Zhang, S. Song, Y. Shu, X. Chen and J. Wang, J. Mater. Chem. B, 2018, 6, 5596
    DOI: 10.1039/C8TB01341A

Search articles by author

Spotlight

Advertisements