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Precise and Label-free Tumour Cell Recognition Based on A Black Phosphorus Nanoquenching Platform

Abstract

Breast cancer is a type of heterogeneous disease which manifests as different molecular subtypes due to the complex nature of tumour initiation, progression, and metastasis. Accurate idenfication of a breast cancer subtype plays crucial roles for breast cancer management. Herein, taking the advantages of efficient quenching properties of black phosphorus nanosheets (BPNSs), in combination with the high specificity of ssDNA (or RNA) aptamer, a fluorometric duplexed assay that is capable of simultaneous detection of two tumour markers within one run is developed. When mixed with BPNSs, the fluorescence of both FAM and Cy3 labelled aptamers was quenched. The presence of different subtypes of breast cancer cells restored the FAM and Cy3 fluoresecence in distinct patterns according to their intrisic features. The proposed assay can precisely recognise label-free breast cancer subtypes, providing an efficient way for cell type identification and guidance for subsequent breast cancer treatment. The significance of the proposed study is two-fold. First we provide a simple way for sensitive and specific tumour cell detection; secondly and more importantly, the proposed dual assay allows precise recognization of tumour cells and thus opens a door for rapid characterization and sorting of a wide range of tumours without using expensive instruments.

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Publication details

The article was received on 14 May 2018, accepted on 01 Aug 2018 and first published on 04 Aug 2018


Article type: Paper
DOI: 10.1039/C8TB01275J
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Precise and Label-free Tumour Cell Recognition Based on A Black Phosphorus Nanoquenching Platform

    W. Yan, X. Wang, J. Yu, X. Meng, P. Qiao, H. Yin, Y. Zhang and P. Wang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01275J

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