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Wheat germ agglutinin modified magnetic iron oxide nanocomplex as cell membrane specific receptor target material for killing breast cancer cells

Abstract

Lectins are known for their specificity for carbohydrate binding. However, few specific carbohydrate residues are over expressed in cancer cells which can be an advantage for using the corresponding lectin. Herein, we report the strategic design of wheat germ agglutinin (WGA) and fluorescent torch, FITC immobilized on NPs of Fe3O4 as cell membrane specific receptor target to breast cancer cells, viz; MCF-7 and MDA-MB-231. The resultant nanocomplexes are well characterized by microscopy and spectroscopy. The WGA tagged nanocomplex is further loaded with anticancer drug, 5- fluorouracil (5-FU) to specifically target and kill the cancer cells. The loading efficiency of 5-FU is ~356 µg/mg. The nanocomplex itself shows ~90% cell viability for all the four cell lines (HEK, HeLa, MCF-7 and MDA-MB-231) studied and therefore is a suitable and targeting drug delivery vehicle. However, the nanocomplex loaded with {WGA+5-FU} shows ~1.5-fold decrease in the cell viability in case of specific cells (MCF-7 and MDA-MB-231) when compared to non-specific cells (HeLa and HEK). The internalization of the nanocomplex is supported by fluorescence microscopy and confocal laser scanning microscopy techniques by tracking with the fluorescent torch, FITC. The nanocomplex internalizes by ~2 times more in specific cells as compared to non- specific cells. It is observed that the internalization is ~2 fold increased when the MDA-MB-231 cells are exposed to magnetic field for 24 h as compared to absence of magnetic field. The live-dead assay of 5-FU loaded nanocomplex was done by propidium iodide (PI) staining. It is seen that there is an increase in cell death to ~2.5 fold when the cells are exposed to magnet as measured from PI uptake. Such strategic designing of nanocomplex can lead to the development of a better method to selectively target and kill the cancer cells by suitable drug delivery carrier, as result of which the medical field can be benefitted.

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Publication details

The article was received on 03 May 2018, accepted on 07 Aug 2018 and first published on 07 Aug 2018


Article type: Paper
DOI: 10.1039/C8TB01170B
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Wheat germ agglutinin modified magnetic iron oxide nanocomplex as cell membrane specific receptor target material for killing breast cancer cells

    C. P. Rao, A. Upadhyay and R. Kandi, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01170B

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