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A Label-Free Aptamer Based Cytosensor for Specific Cervical Cancer HeLa Cell Recognition through g-C3N4-AgI/ITO Photoelectrode

Abstract

Facile and efficient detection of cancer cells at the early phases of a disease is one of the main challenges in the cancer diagnostics. It is found that photoactive materials and bio-recognition elements are two key factors for a promising photoelectrochemical (PEC) biosensor for cancer cell detection which could play a significant role for realizing the cancer early diagnostics with high sensitivity and selectivity. In this study, we designed a novel label-free PEC aptamer based cytosensor for the specific detection of cancer cells like Hela cells by using water-dispersible g-C3N4-AgI nanocomposites as visible light-sensitive material and anti-CEM/PTK7 aptamer as the bio-recognition element. It is observed that when a suitable amount of AgI nanoparticles were doped in the two-dimensional graphite-like carbon nitride nano-sheets (g-C3N4 NSs), the visible light photocurrent response could be significantly improved. The PEC response of the as-prepared biosensor based on g-C3N4-AgI/ITO photoelectrode was linearly proportional to the relevant cancer cells like Hela cells in the concentration ranging from 10 to 106 cells/mL with a limit of detection of 5 cells/mL. In addition, the g-C3N4-AgI/ITO photoelectrode and the fabricated cytosensor exhibit long-term stability, good reproducibility, excellent selectivity, and high sensitivity, demonstrating the successful conjugation of g-C3N4-AgI NSs with aptamer and target cancer cells in the high performance PEC cytosensor.

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Publication details

The article was received on 23 Apr 2018, accepted on 09 Jul 2018 and first published on 12 Jul 2018


Article type: Paper
DOI: 10.1039/C8TB01067F
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    A Label-Free Aptamer Based Cytosensor for Specific Cervical Cancer HeLa Cell Recognition through g-C3N4-AgI/ITO Photoelectrode

    R. Motaghed Mazhabi, L. Ge, H. Jiang and X. Wang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01067F

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