Jump to main content
Jump to site search


A Simple Strategy for Robust Hydrogel Preparation and Characterisation Derived from Chitosan and Amino Functional Monomers for Biomedical Applications

Abstract

Molecular interactions of amino functional (AF) monomers with chitosan (CS) lead to the formation of external stimuli responsive hydrogels (HGs). These have the potential to produce biomaterials with novel properties in a simple blending approach. Six independent AF monomers such as diethylenetriamine (DETA), bis(3-aminopropyle)amine (BAPA), 3,3'-diamino-N-methyldipropyleamine (DAMPA), hexamethylenediamine (HMDA), N,N-dimethylethylamine (DMEA) and diethylamine (DEA) with distinct functional groups and chain lengths were designed to form stable HGs at physiological pH. Such AF monomers are able to form HG within a short time (in the range from 10 to 19 seconds) via physically interacting with CS. This is an alternative to the covalently crosslinking reaction process, providing cost effective HG biomaterials. HG complexes were characterized by rheometer, differential scanning calorimeter (DSC), and Fourier transform infrared (FTIR) spectroscopy. The interaction between AF monomers and CS polymer has been discussed and the results have been confirmed by FTIR analysis. Storage modulus (G'), loss modulus (G") and complex viscosity (η*) were evaluated for all HGs using a rheometer, and optimized the ratios of CS and particular AF monomer for stable HG formation. Swelling ratio was evaluated using a simple method, and was found to be directly related to the structure of the AF monomer, pH and temperature. These HGs were utilised for encapsulation, and release of active molecules (e.g., reactive red 120 (RR120) as a model compound) as measured at low pH 5.5, physiological pH 7.4 and high pH 9.5. The cell viability and cellular compatibility of the HGs were evaluated in vitro cell culture, demonstrating that all the five different types of HGs support cellular compatibility, attachment and growth. The physical mixing of AF monomers with CS is expedited new bespoke economically viable biomaterials development.

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Mar 2018, accepted on 10 Jul 2018 and first published on 12 Jul 2018


Article type: Paper
DOI: 10.1039/C8TB00865E
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
  •   Request permissions

    A Simple Strategy for Robust Hydrogel Preparation and Characterisation Derived from Chitosan and Amino Functional Monomers for Biomedical Applications

    F. Khan, F. Aratsu, S. Kobayashi and M. Tanaka, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00865E

Search articles by author

Spotlight

Advertisements