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Issue 28, 2018
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Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells

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Abstract

Hematopoietic stem cells are used in transplantations for patients with hematologic malignancies. Scarce sources require efficient strategies of expansion, including polymeric biomaterials mimicking architectures of bone marrow tissue. Tissue microenvironment and mode of cytokine presentation strongly influence cell fate. Although several cytokines with different functions as soluble or membrane-bound mediators have already been identified, their precise roles have not yet been clarified. A need exists for in vitro systems that mimic the in vivo situation to enable such studies. One way is to establish surfaces mimicking physiological presentation using protein-immobilization onto polymer films. However these films merely provide a static presentation of the immobilized proteins. It would be advantageous to also dynamically change protein presentation and functionality to better reflect the in vivo conditions. The electroactive polymer polypyrrole shows excellent biocompatibility and electrochemically alters its surface properties, becoming an interesting choice for such setups. Here, we present an in vitro system for switchable presentation of membrane-bound cytokines. We use interleukin IL-3, known to affect hematopoiesis, and show that when immobilized on polypyrrole films, IL-3 is bioavailable for the bone marrow-derived FDC-P1 progenitor cell line. Moreover, IL-3 presentation can be successfully altered by changing the redox state of the film, in turn influencing FDC-P1 cell viability. This novel in vitro system provides a valuable tool for stimuli-responsive switchable protein presentation allowing the dissection of relevant mediators in stem and progenitor cell behavior.

Graphical abstract: Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells

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Supplementary files

Article information


Submitted
21 Mar 2018
Accepted
25 Jun 2018
First published
02 Jul 2018

This article is Open Access

J. Mater. Chem. B, 2018,6, 4665-4675
Article type
Paper

Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells

J. Baumgartner, J. Jönsson and E. W. H. Jager, J. Mater. Chem. B, 2018, 6, 4665
DOI: 10.1039/C8TB00782A

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